Beef spoilage is primarily caused by bacteria, and maximizing shelf-life is dependent on a multiple hurdle approach to generate conditions that inhibit microbial growth. This chapter begins by reviewing the factors that affect beef spoilage and microbial shelf-life, and the bacteria and processes involved in spoilage. Subsequent sections then consider methods for maximizing the microbial shelf-life of beef products, including minimizing the initial microbial burden in beef, chilling of beef carcasses and the wide variety of packaging technologies