This chapter aims to address the environmental aspects of greenhouse cultivation, it covers the methodology used to perform an environmental assessment and provides information regarding damaging and beneficial environmental aspects, as well as suggestions to improve environmental quantification methods for greenhouse production systems. The chapter explores the methodology used to account for environmental impacts using a life cycle perspective, including a brief summary of life cycle assessment (LCA) tools, and its application to greenhouse crop production. The chapter describes the different components involved in greenhouse cultivation (including structures, greenhouse management, fertilization, pesticides and waste management) and how to include them in the environmental quantification assessment. Finally, the chapter draws conclusions regarding not only the improvement of environmental aspects of greenhouse cultivation but also enhancement of environmental quantification tools.