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This edited book is written for students, postdocs and established investigators who want to enter the field of single-particle cryo-EM. This is a recently developed method to determine high-resolution structures of biological macromolecules. A major strength is the fact that cryo-EM does not require prior crystallization of protein complexes. Furthermore, it is especially well suited for larger complexes and molecular machines, although it can also be used for macromolecules as small as 40 kDa or less. It also is especially well-suited to deliver high-resolution structural information about multiple conformational states.Key Features
- Written for students, postdocs and established investigators who want to enter the field of single-particle cryo-EM.
- Provides a comprehensive, accessible and authoritative introduction to the field of high-resolution structure analysis by single-article cryo-EM.
- Covers all necessary background, ranging from the underlying concepts to practical aspects such as specimen preparation, data-collection, data analysis, and the final validation of results.
This is the book many people have been asking for - a comprehensive account of the theory and practice of single cryo-electron microscopy (cryo-EM), the imaging method used to determine the three-dimensional (3D) structures of biological macromolecules and their assemblies. Cryo-EM has been through an explosive period of development to become a prominent approach in structural biology. Its dramatic advances have attracted crystallographers, biochemists and molecular biologists who were previously sceptical or unaware of the power of the method, to apply it to their problems. There is a great demand for a comprehensive documentation of the theory and practice in a single reference text, rather than a profusion of methods articles and brief reviews.
Helen Saibil, Crystallography News, September 2022
— Helen Saibil
Professor Glaeser (UC Berkeley) made major contributions to the initial development of cryo-EM technology, and he currently is active in the improvement of specimen preparation.
Professor Nogales (UC Berkeley) has used cryo-EM throughout her scientific career, successfully applying this technique for the last three decades to systems that defied structural characterization.
Professor Chiu (Stanford University) developed early experimental and computational methods towards 3D structure determination of biomolecules toward atomic resolution.