‘Nanobiotechnology, basic and applied aspects’ is expected to be of tremendous value to the group of scientists, involved in both basic and applied biology and engineering. The proposed book is a comprehensive compendium of basics of nanoscience and its application in biophysical and biomedical problems. The book describes a brief history and evolution of nanoscience in the first two chapters, which is interesting, and an enriched resource for the undergraduates of nanotechnology and biotechnology. The subsequent chapters gives an in-depth idea of different nanomaterials and their diverse biological applications such as bio-imaging, drug-development, drug-delivery, biosensors etc.. The book could also be immensely interesting for the geologists and naturalists, since it reports the occurrence of nanoparticles, which are derived from biological samples of human, and plants or of edaphic origin. In summary, the book proposed could be a reference or ready-reckoner in the undergraduate/college course-works in nanoscience and nano-biotechnology. It also gives a clear idea of different research directions in the field of nanobiotechnology.