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This book is a comprehensive guide to the intricate world of assisted reproductive technology (ART), offering both foundational knowledge and advanced techniques in embryology.
Covering a wide range of topics, from laboratory set-up and semen analysis to advanced procedures such as intracytoplasmic sperm injection (ICSI) and embryo biopsy, this book provides invaluable insights for both experienced professionals and those new to the field.
Authored by experts with extensive experience in the field of embryology, the text focuses on practical applications, techniques and best practices, serving as an essential resource for anyone involved in ART.
Chandan Vijay Salunke PhD
Managing Director, Kasturi ReproAssist Concepts LLP, Panjim, Goa, India
Shravani Kanakdas Parker MSc
Executive Director, Kasturi ReproAssist Concepts LLP, Panjim, Goa, India
1. Introduction to Assisted Reproductive Technology Laboratory
2. Setting Up an Assisted Reproductive Technology Laboratory
3. Quality Control in Assisted Reproductive Technology Laboratory
4. Semen Analysis According to World Health Organization 2021 Guidelines
5. Sperm Preparation and Cryopreservation
6. Culture Systems and Media
7. Oocyte Retrieval
8. In Vitro Fertilization
9. Oocyte Denudation
10. Oocyte Grading
11. Intracytoplasmic Sperm Injection
12. Fertilization Assessment and Embryo Grading
13. Selection of Embryos for Transfer
14. Blastocyst Culture and Transfer
15. Assisted Hatching
16. Embryo Biopsy and Preimplantation Genetic Diagnosis
17. Vitrification and Thawing of Oocytes and Embryos
18. Ethical and Legal Considerations in Assisted Reproductive Technology
19. Future Trends in Assisted Reproductive Technology