Safety and biosecurity of animals, particularly farm animals and workers, are a major concern for agricultural industry as well as public health. Designing and implementing effective biosecurity measures are crucial for preventing the spread of animal diseases and controlling zoonotic disease to humans through a range of practical and strategic approaches. The principal goals of this chapter are to reduce the risk of disease transmission to chicks and/or workers by following to established poultry farming safety standards, specifically utilizing appropriate techniques, and maintaining continuous monitoring.