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Advances in soil quantitative polymerase chain reaction (qPCR) techniques for identifying and studying soil microbial communities
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04 February 2026

This chapter explores advances in quantitative PCR (qPCR) techniques for identifying and studying soil microbial communities. The authors begin by describing the principles of qPCR. This is followed by sections discussing factors affecting the reproducibility of qPCR assays, and qPCR targets. They then explain linking soil communities to measured processes. This is then followed by two sections covering case studies and recent developments.
TECHNOLOGY & ENGINEERING / Agriculture / Agronomy / Soil Science, TECHNOLOGY & ENGINEERING / Agriculture / Sustainable Agriculture, TECHNOLOGY & ENGINEERING / Agriculture / Agronomy / Crop Science
- 1 Introduction
- 2 Principles of qPCR
- 3 Factors affecting the reproducibility of qPCR assays
- 4 qPCR targets
- 5 Linking soil communities to measured processes
- 6 Case studies of the use of qPCR for measuring soilhealth
- 7 Recent developments
- 8 Conclusion
- 9 References