Dr Marta Vázquez Vilar

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  • Advances in assembling gRNA/Cas9 constructs in genome editing of plants
    Advances in assembling gRNA/Cas9 constructs in genome editing of plants

    Advances in assembling grna/cas9 constructs in genome editing of plants

    This chapter reviews the principles of CRISPR cloning in binary vectors and the different methods and elements employed, including the nucleases alternative to Cas9. It pays special attention to modular cloning strategies and multiplexing tools as well as the engineering of expanded Cas activitie...

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