The dry period lays a foundation for a successful next lactation, especially from the udder health perspective. It is a high-risk period for acquiring new intramammary infections (IMI), but it also provides an excellent opportunity for eliminating existing subclinical infections. The way cows are dried off and milking is halted at the end of lactation impacts the involution process, mammary health and cow comfort. Antibiotic dry cow therapy (DCT) has played a crucial part in mastitis control, but due to global concerns about increasing antibiotic resistance, the approach to DCT is evolving. This chapter reviews the current knowledge about the impact of milk cessation methods (abrupt vs. gradual dry-off) on mammary involution, udder health and cow comfort. The importance of dry cow therapy is discussed, especially in the light of current global concerns related to antibiotic resistance.